1270 Non-invasive imaging reveals keratinocyte-melanocyte changes in treatment-resistant vitiligo skin
نویسندگان
چکیده
Vitiligo is an autoimmune skin disease characterized by the progressive destruction of melanocytes autoreactive T cells, resulting in persistent white patches. We previously combined multiphoton microscopy (MPM) and single cell RNA sequencing (scRNA-seq) stable vitiligo lesions to identify a subset metabolically shifted keratinocytes, which we now call ‘stable keratinocytes’ (SVKs), that accumulate skin. SVKs produce high levels chemokines known induce (CXCL9, CXCL10) low Wnt signals recruit melanocytes. were associated with persistence, as they not observed acute vitiligo. also noted less numerous from patients responded punch grafting treatment compared failed respond. To further investigate role resistance, followed 15 treated over time using two live imaging methods – MPM reflectance confocal (RCM), has wider field view allows us better visualize migrating persisted up 6 weeks responders but abundant after 10 weeks. In resistant patients, past Using RCM, showed could be visualized surrounding grafts early 3 contrast, no did respond treatment. summary, our work indicates are more fail therapy, suggesting presence may biomarker for would therapy.
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2023
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2023.03.1284